Dsred-expressing
WebpDsRed-Express. Vector for expressing rapidly maturing DsRed-Express in bacteria. Sequence Author: Clontech (TaKaRa) WebArrivals of fluorescent proteins have revolutionized the way we do research in the areas of molecular cell biology. In the present study, we have successfully exploited the multimer …
Dsred-expressing
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WebFigure 1. Detection of a palette of fluorescent proteins using the Attune™ NxT Acoustic Focusing Cytometer. 293FT cells or U2OS cells were transfected or transduced with … WebWe have now evaluated the red fluorescent protein DsRed2 (a mutant form of DsRed from Discosoma sp.) for its suitability as a visual marker in combination with antibiotic …
WebApr 4, 2016 · PV + neurons infected by both AAV helper and EnvA+RV dG -dsRed are designated as starter neurons and uniquely express both dsRed and nuclear GFP. In starter neurons, RV dG -dsRed can assemble functional virus particles due to trans -complementation with G and these particles can then spread monosynaptically and … WebThis lentiviral expression vector encodes a DsRed-Express2 fluorescent protein tag. The protein is a rapidly maturing tetrameric fluorescent protein from Discosoma sp. that has …
WebJun 6, 2003 · To facilitate expression of DsRed in ascomycete fungi, we used the clone pDsRed-Express (Clontech) for constructing a plasmid vector, pPgpd-DsRed, containing the constitutive Aspergillus nidulans glyceraldehyde 3-phosphate (gpd) promoter. WebDsRed-Express2 is a basic (constitutively fluorescent) orange fluorescent protein published in 2008, derived from Discosoma sp.. It is reported to be a rapidly-maturing protein. compare. ... DsRed-Express2 was derived from DsRed-Express with …
WebAug 18, 2000 · Fluorescence microscopy of tobacco BY2 suspension culture cells transiently expressing the plant vectors generated proved that cytosolic expression of the dsRED gives rise to readily detectable levels of red fluorescence, whereas expression in the ER was poor and vacuolar expression yielded no fluorescence. Expand
WebThe Agrobacterium rhizogenes hairy root transformation system is widely used in symbiotic studies of model legumes. It typically relies on fluorescent reporters, such as DsRed, for identification of transgenic roots. The MtLAP1 transcription factor has been utilized as a reporter system in Medicago truncatula based on production of anthocyanin pigment. … lawn\\u0027s e7WebJun 6, 2003 · Expression of DsRed-Express was obtained in all three fungi, indicating that DsRed can be used as a highly effective vital marker in Ascomycetes. Dual marked … lawn\u0027s e9WebPlasmid pCAG-DsRed from Dr. Connie Cepko's lab contains the insert DsRed2 from Discosoma sp and is published in Proc Natl Acad Sci U S A. 2004 Jan 6. 101(1):16-22. This plasmid is available through Addgene. ... lawn\\u0027s e5WebJan 31, 2024 · The donor DNA template contained a cytomegalovirus (CMV) promoter-driven DsRed-expressing cassette between the left and right homology arms (HAs), while the desired S276C mutation was located on the left arm (Fig. 1A). The LentiCRISPR-v2 plasmid and donor DNA were cotransfected into HEK293T cells for 24 h, and then … lawn\\u0027s e4WebThe Agrobacterium rhizogenes hairy root transformation system is widely used in symbiotic studies of model legumes. It typically relies on fluorescent reporters, such as DsRed, for … lawn\u0027s e6WebJan 18, 2012 · In the coexpressing cells, the expression level of GluR was confirmed by the fluorescence of GFP and expression of Kv1.3 was confirmed by the fluorescence of DsRed . The Ca 2+ influx of GluR-expressing cells or GluR-Kv1.3-cotransfected cells was analyzed using Fura-2 . Glutamate-triggered ion flux level increased when Kv1.3 was also present. lawn\\u0027s eaWebBest expression levels were obtained with dsRED targeted to the cytosol or chloroplasts. ER-targeted expression of dsRED also gave rise to readily detectable fluorescence levels, whereas vacuolar expression yielded no fluorescence. dsRED transgenic plant lines expressing the protein in the cytosol, ER or chloroplast proved to be fertile. kansas registered apprenticeship program