WebGastrointestinal stem cells can be propagated by using conditioned medium from a supportive cell line (L-WRN). This protocol describes how to prepare conditioned medium and how to culture stem cell-enriched epithelial spheroids from the mouse gastrointestine. These spheroids are also amenable to genetic modification with recombinant lentiviruses. Webl-wrn小鼠皮下结缔组织细胞. 产品编号:ys1109c 产品类别:小鼠细胞系 生长特性:贴壁生长 培养体系:dmem(高糖)+10%fbs 传代方法 :1:2传代 细胞形态:成纤维细胞样 …
L-WRN conditioned medium for gastrointestinal epithelial stem …
WebL-WRN细胞ATCC CRL-3276小鼠皮下结缔组织细胞培养操作. 1)复苏细胞:将含有 1mL 细胞悬液的冻存管在 37℃水浴中迅速摇晃解冻,加 入 4mL 培养基混合均匀。. 在 … WebThe 3dGRO ™ L-WRN Conditioned Media Supplement should be stored at -20°C. When ready to use, thaw the L-WRN Conditioned Medium overnight at 2-8°C in the dark. Thawed medium is good for two weeks when stored at 2-8°C. If media will not be used within two weeks, aliquot unused media and store at -20°C. After thawing refrozen aliquots, do ... footprints background verification company
S型铂铑热电偶WRP-130型0-1600度WRN-132/122刚玉陶瓷管1300度K型 K型L…
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