WebOct 28, 2024 · Reporter genes eGFP and RFP, linked by either P2A or T2A, were cloned into the polyclonal site to construct the CMV-eGFP-P2A-RFP (P2A) and CMV-eGFP-T2A-RFP (T2A) vectors (Fig. 1a). The P2A and T2A DNA sequences were synthesized by General Biosystems Company (Chuzhou, China). WebHigh-risk melanoma usually is defined as melanoma that is deeper or thicker (more than 4.0 millimeters thick) at the primary site or involves nearby lymph nodes. This disease has a high risk of recurrence because some melanoma cells may remain in the body even after the surgery removed the visible melanoma tumors successfully.
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Generation of Brachyury-mCherry knock-in reporter human pluripotent …
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