網頁A "Buffy Coat", also known as leukocyte concentrate, is a byproduct in the manufacturing of red blood cell and platelet concentrate from a whole blood donation. The compatibility of the donated blood samples are increased due to the removal of leukocytes that are known to belong to the human immune system, increases the compatibility and the ... 網頁2024年10月4日 · To isolate PBMCs, the buffy coat is extracted, washed with salt-buffered solution, and then centrifuged allowing the cells to be recovered with high yield in a small volume. The supernatant, containing platelets, separation medium, and plasma, is removed, leaving a pellet of purified PBMCs.
What Exactly Is A Buffy Coat? Cytologics
網頁1997年8月1日 · A study of buffy coats stored in various media and containers at 22°C suggests that it is better to restrict storage to 24 h or less to avoid activation or other deleterious effects on the platelets. Background: Buffy coats (BCs) are used as an alternative to platelet‐rich plasma in the preparation of platelet concentrates (PCs). For … 網頁Buffy coat preparation is often the first step towards additional processing for T-cell depletion, tumor cell purging or cryopreservation for both marrow and blood HPC collections. A white blood cell-rich concentrate, the buffy coat, is prepared by centrifuging the original product using a standard blood bank centrifuge, a blood cell washer, or ... photo cocker anglais
An Efficient Culture Method of CD3-Positive T Cells from Human Cryopreserved Buffy Coat …
網頁2013年10月1日 · Some molecular analyses require microgram quantities of DNA, yet many epidemiologic studies preserve only the buffy coat. In Frederick, Maryland, in 2010, we estimated DNA yields from 5 mL of whole blood and from equivalent amounts of all-cell-pellet (ACP) fraction, buffy coat, and residual blood cells from fresh blood (n = 10 … The buffy coat is commonly used for DNA extraction, [4] with white blood cells providing approximately 10 times more concentrated sources of nucleated cells. [5] They are extracted from the blood of mammals because mammalian red blood cells are anucleate and do not contain DNA. A common protocol is … 查看更多內容 The buffy coat is the fraction of an anticoagulated blood sample that contains most of the white blood cells and platelets following centrifugation. 查看更多內容 After centrifugation, one can distinguish a layer of clear fluid (the plasma), a layer of red fluid containing most of the red blood cells, … 查看更多內容 • Leukocytes • Ficoll • Malaria 查看更多內容 Quantitative buffy coat (QBC), based on the centrifugal stratification of blood components, is a laboratory test for the detection of … 查看更多內容 • Blood Buffy Coat at the U.S. National Library of Medicine Medical Subject Headings (MeSH) 查看更多內容 網頁The buffy coat accounts for less than 1 percent of a whole blood sample, yet it contains the majority of white blood cells (WBC) and platelets. In fact, leukocytes can be 10-20X more concentrated in the buffy coat than in … photo cochise